Hum noise such as power line interference is one of the critical problems in the biomedical signal acquisition. Various techniques have been proposed to suppress power line interference. However, some of the techniques require more components and power consumption. The notch depth in the conventional N-path notch filter circuits needs a higher number of paths and switches off-resistance. It makes the conventional N-path notch filter less of efficiency to suppress hum noise. This work proposed the new N-path notch filter to hum noise suppression in biomedical signal acquisition. The new N-path notch filter achieved notch depth above 40dB with sampling frequency 50Hz and 60Hz. Although the proposed circuits use less number of path and switches off-resistance. The proposed circuit has been verified using artificial ECG signal contaminated by hum noise at frequency 50Hz and 60Hz. The output of N-path notch filter achieved a noise-free signal even if the sampling frequency changes.