Target location estimation is one of many promising applications of wireless sensor networks. However, until now only few studies have examined location estimation performances in real environments. In this paper, we analyze the effect of walking people on target location estimation performance in three experimental locations. The location estimation is based on received signal strength indicator (RSSI) and maximum likelihood (ML) estimation, and the experimental locations are a corridor of a shopping center, a foyer of a conference center and a laboratory room. The results show that walking people have a positive effect on the location estimation performance if the number of RSSI measurements used in the ML estimation is equal or greater than 3, 2 and 2 in the case of the experiments conducted in the corridor, foyer and laboratory room, respectively. The target location estimation accuracy ranged between 2.8 and 2.3 meters, 2.5 and 2.1 meters, and 1.5 and 1.4 meters in the case of the corridor, foyer and laboratory room, respectively.