An enzymatic biofuel cell (BFC) that uses lactic acid in human sweat as fuel to generate electricity is an attractive power source for wearable devices. A BFC capable of generating electricity with human sweat has been developed. It comprised a flexible tattoo seal type battery with silver oxide vapor deposited on a flexible material and conductive carbon nanotubes printed on it. The anode and cathode in this battery were arranged in a plane (planar type). This work proposes a thin laminated enzymatic BFC by inserting a cellulose nanofiber (CNF) sheet between two electrodes to absorb human sweat (stack-type). Optimization of the anode and changing the arrangement of electrodes from planar to stack type improved the output and battery life. The stack type is 43.20μW / cm2 at 180mV, which is 1.25 times the maximum power density of the planar type.

Conventional enzymatic biofuel cells (EBFCs) use glucose solution or glucose from human body. It is desirable to get glucose from a substance containing glucose because the glucose concentration can be kept at the optimum level. This work developed a biofuel cell that generates electricity from cellulose, which is the main components of plants, by using decomposing enzyme of cellulase. Cellulose nanofiber (CNF) was chosen for the ease of decomposability. It was confirmed by the cyclic voltammetry method that cellulase was effective against CNF. The maximum output of the optimized proposed method was 38.7 μW/cm2, which was 85% of the output by using the glucose solution at the optimized concentration.

In this study, we devised a biofuel cell (BFC) by impregnating sheet-like cellulose nanofiber (CNF) with liquid fuel (fructose) and sandwiching it with the electrodes, making the structure simple and compact. CNF was considered as a suitable material for BFC because it is biocompatible, has a large specific surface area, and exhibits excellent properties as a catalyst and an adsorbent. In this BFC device, graphene-coated carbon fiber woven cloth (GCFC) was used as the material for preparing the electrodes, and the amount of enzyme modification on the surface of each electrode was enhanced. Further, as the distance between the electrodes was same as the thickness of the sheet-shaped CNF, it facilitated the exchange of protons between the electrodes. Moreover, the cathode, which requires an oxidation reaction, was exposed to the atmosphere to enhance the oxygen uptake. The maximum power density of the CNF-type BFC was recorded as 114.5 µW/cm2 at a voltage of 293 mV. This is more than 1.5 times higher than that of the liquid-fuel-type BFC. When measured after 24 h, the maximum power density was recorded as 44.9 µW/cm2 at 236 mV, and the output was maintained at 39% of that observed at the beginning of the measurement. However, it is not the case with general BFCs, where the power generation after 24 h is less than 5%. Therefore, the CNF-type BFCs have a longer lifespan and are fuel efficient.