Accurate and low-cost sensor localization is critical for deployment of wireless sensor networks. Distance between Ultra-wideband (UWB) sensor nodes can be obtained by measuring round trip flying time through two-way ranging (TWR) transaction. Because of difficulties in synchronization and channel estimate, the response delay of UWB node is the order of milliseconds. Comparing with the nanosecond propagation delay, relative clock offset between UWB nodes introduces big error in TWR. This paper presents the management of relative clock offset in TWR transaction. The relative clock offset between sensors is estimated by comparing the claimed and real frame duration. Simulation in the UWB channel model shows the relative clock offset after compensation can be reduced to less than 2 ppm.