Rapid Bacterial Testing Method by Size Distribution Measurement with Laser Light Scattering

Hajime HASHIMOTO; Koji KAKIHARA; Hidetoshi MIIKE; Yoshio EBINA

Rapid Bacterial Testing Method by Size Distribution Measurement with Laser Light Scattering

Hajime HASHIMOTO, Koji KAKIHARA, Hidetoshi MIIKE, Yoshio EBINA

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A rapid testing is required in clinical laboratory. A new method for rapid bacterial testing is proposed by size distribution measurement with laser light scattering. Bacterial sample is suspended in a saline solution and the scattering pattern is measured from 5 to 90 degrees with 5-degree intervals. The size distribution function is calculated from the light scattering pattern by the inversion technique based on the modified Rayleigh-Debye approximation. The difference in bacterial species and the influences of drug are investigated. The following results are obtained; (1) size distribution curve reflects the morphological characteristics of bacteria, and bacterial species are discriminated roughly by two characteristic parameters (mean size and dispersion width) obtained from the curve; (2) size distribution pattern changes in a short time (within one hour) indicating the effects of drug. It is useful for antibiotics susceptibility testing.

Publication: IEICE TRANSACTIONS on transactions Vol.E68-E No.5 pp.304-308

Publication Date: 1985/05/25

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Type of Manuscript: PAPER

Category: Optical and Quantum Electronics

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Hajime HASHIMOTO
Koji KAKIHARA
Hidetoshi MIIKE
Yoshio EBINA

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Hajime HASHIMOTO, Koji KAKIHARA, Hidetoshi MIIKE, Yoshio EBINA, "Rapid Bacterial Testing Method by Size Distribution Measurement with Laser Light Scattering" in IEICE TRANSACTIONS on transactions, vol. E68-E, no. 5, pp. 304-308, May 1985, doi: .
Abstract: A rapid testing is required in clinical laboratory. A new method for rapid bacterial testing is proposed by size distribution measurement with laser light scattering. Bacterial sample is suspended in a saline solution and the scattering pattern is measured from 5 to 90 degrees with 5-degree intervals. The size distribution function is calculated from the light scattering pattern by the inversion technique based on the modified Rayleigh-Debye approximation. The difference in bacterial species and the influences of drug are investigated. The following results are obtained; (1) size distribution curve reflects the morphological characteristics of bacteria, and bacterial species are discriminated roughly by two characteristic parameters (mean size and dispersion width) obtained from the curve; (2) size distribution pattern changes in a short time (within one hour) indicating the effects of drug. It is useful for antibiotics susceptibility testing.
URL: https://globals.ieice.org/en_transactions/transactions/10.1587/e68-e_5_304/_p

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@ARTICLE{e68-e_5_304,
author={Hajime HASHIMOTO, Koji KAKIHARA, Hidetoshi MIIKE, Yoshio EBINA, },
journal={IEICE TRANSACTIONS on transactions},
title={Rapid Bacterial Testing Method by Size Distribution Measurement with Laser Light Scattering},
year={1985},
volume={E68-E},
number={5},
pages={304-308},
abstract={A rapid testing is required in clinical laboratory. A new method for rapid bacterial testing is proposed by size distribution measurement with laser light scattering. Bacterial sample is suspended in a saline solution and the scattering pattern is measured from 5 to 90 degrees with 5-degree intervals. The size distribution function is calculated from the light scattering pattern by the inversion technique based on the modified Rayleigh-Debye approximation. The difference in bacterial species and the influences of drug are investigated. The following results are obtained; (1) size distribution curve reflects the morphological characteristics of bacteria, and bacterial species are discriminated roughly by two characteristic parameters (mean size and dispersion width) obtained from the curve; (2) size distribution pattern changes in a short time (within one hour) indicating the effects of drug. It is useful for antibiotics susceptibility testing.},
keywords={},
doi={},
ISSN={},
month={May},}

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TY - JOUR
TI - Rapid Bacterial Testing Method by Size Distribution Measurement with Laser Light Scattering
T2 - IEICE TRANSACTIONS on transactions
SP - 304
EP - 308
AU - Hajime HASHIMOTO
AU - Koji KAKIHARA
AU - Hidetoshi MIIKE
AU - Yoshio EBINA
PY - 1985
DO -
JO - IEICE TRANSACTIONS on transactions
SN -
VL - E68-E
IS - 5
JA - IEICE TRANSACTIONS on transactions
Y1 - May 1985
AB - A rapid testing is required in clinical laboratory. A new method for rapid bacterial testing is proposed by size distribution measurement with laser light scattering. Bacterial sample is suspended in a saline solution and the scattering pattern is measured from 5 to 90 degrees with 5-degree intervals. The size distribution function is calculated from the light scattering pattern by the inversion technique based on the modified Rayleigh-Debye approximation. The difference in bacterial species and the influences of drug are investigated. The following results are obtained; (1) size distribution curve reflects the morphological characteristics of bacteria, and bacterial species are discriminated roughly by two characteristic parameters (mean size and dispersion width) obtained from the curve; (2) size distribution pattern changes in a short time (within one hour) indicating the effects of drug. It is useful for antibiotics susceptibility testing.
ER -