This paper proposes single antenna relay system using De-noise and forward (DNF) scheme for MIMO transmission. In this scheme, the relay node eliminates the noise by identifying constellation, and retransmits after amplification. DNF does not amplify the noise, and the channel information is unnecessary in the transmitting side. In this paper, we propose the de-noising scheme for MIMO application. Particularly, DNF can be used for the multi-stream transmission even though each relay nodes have single antenna. The simulation demonstrates the proposed scheme can improve the data transmission quality than the conventional scheme.