This paper presents a pilot-aided channel estimation method which is particularly suitable for mobile WiMAX 802.16e Downlink Partial Usage of Subchannel mode. Based on this mode, several commonly used channel estimation methods are studied and the method of least squares line fitting is proposed. As data of users are distributed onto permuted clusters of subcarriers in the transmitted OFDMA symbol, the proposed channel estimation method utilizes these advantages to provide better performance than conventional approaches while offering remarkably low complexity in practical implementation. Simulation results with different ITU-channels for mobile environments show that depending on situations, enhancement of 5 dB or more in term of SNR can be achieved.